Digoxigenin-labelled molecular probe for the simultaneous detection of three potato pathogens: potato spindle tuber viroid (PSTVd), potato virus Y (PVY), and potato leafroll virus (PLRV).
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چکیده
منابع مشابه
Digoxigenin-labelled molecular probe for the simultaneous detection of three potato pathogens: potato spindle tuber viroid (PSTVd), potato virus Y (PVY), and potato leafroll virus (PLRV).
A molecular probe, p3POT, was constructed of PSTVd, PVY, PLRV cDNA fragments introduced into pUC18 vector. Sequencing of the inserts revealed that cloned fragments covered conservative parts of pathogenic genomes. Dot-blot hybridization of digoxigenin-labelled construct to crude extracts from plants infected with different potato viruses proved high sensitivity and specificity of the p3POT prob...
متن کاملInsight on Genes Affecting Tuber Development in Potato upon Potato spindle tuber viroid (PSTVd) Infection.
Potato (Solanum tuberosum L) is a natural host of Potato spindle tuber viroid (PSTVd) which can cause characteristic symptoms on developing plants including stunting phenotype and distortion of leaves and tubers. PSTVd is the type species of the family Pospiviroidae, and can replicate in the nucleus and move systemically throughout the plant. It is not well understood how the viroid can affect ...
متن کاملتعیین برخی خصوصیات بیولوژیکی و مولکولی potato leafroll virus و potato virus a در استان اصفهان
چکیده سیب زمینی یکی از مهم ترین محصولات زراعی در جهان می باشد. ویروس های متعددی این گیاه را تحت تاثیر قرار می دهند و باعث خسارت اقتصادی قابل توجهی می شوند. ویروس پیچیدگی برگ سیب زمینی potato leafroll virus (plrv) عضو تیپ جنس polerovirus و خانواده luteoviridae و ویروس ای سیب زمینی potato virus a (pva) عضو تیپ جنس potyvirus از خانواده potyviridae شایع ترین ویروس های سیب زمینی می باشند که توسط ش...
15 صفحه اولErratum: The Effects of High Temperature on Infection by Potato virus Y, Potato virus A, and Potato leafroll virus
[This corrects the article on p. 321 in vol. 32, PMID: 27493607.].
متن کاملPotato spindle tuber viroid: the simplicity paradox resolved?
TAXONOMY Potato spindle tuber viroid (PSTVd) is the type species of the genus Posipiviroid, family Pospiviroidae. An absence of hammerhead ribozymes and the presence of a 'central conserved region' distinguish PSTVd and related viroids from members of a second viroid family, the Avsunviroidae. PHYSICAL PROPERTIES Viroids are small, unencapsidated, circular, single-stranded RNA molecules which...
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ژورنال
عنوان ژورنال: Acta Biochimica Polonica
سال: 1994
ISSN: 1734-154X,0001-527X
DOI: 10.18388/abp.1994_4702